MASS SPECTROSCOPY- A VERSATILE TOOL FOR CHARARCTERISING THE LIPID ENVIRONMENT FOR MEMBRANE PROTEIN ASEEMBLIES

Veneeta Anand Harleen Kaur

Abstract


Abstract:- Biological membranes are selectively permeable barriers important for cell organization and compartmentalization. Their organization strongly depends on the lipids that constitute the lipid bilayer as well as the proteins that reside in the membrane. Unraveling the organization of biological membranes is therefore of great importance to understand cellular function driven by integral and peripheral membrane proteins. Recent developments in mass spectrometry made it a powerful tool contributing to our present-day understanding of membrane composition and organization.

The two main deliverables of mass spectrometry are:-

(i)    the identification and quantification of the membrane components.

(ii)   the analysis of their structural arrangements.

In this review article, we first briefly discuss the aspects of membrane organization that are accessible through mass spectrometry. We then provide detailed insights into the various mass spectrometric strategies which help identifying lipids from membranes or membrane protein assemblies, unraveling the lipid binding modes in membrane proteins and uncovering their structural roles. 


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References


N.P. Barrera, C.V. RobinsonAdvances in the mass spectrometry of membrane proteins: from individual proteins to intact complexes Annu. Rev. Biochem., 80 (2011).

N.P. Barrera, M. Zhou, C.V. RobinsonThe role of lipids in defining membrane protein interactions: insights from mass spectrometry Trends Cell Biol., 23 (2013).

C.C. Bechara, C.V. Robinson Different modes of lipid binding to membrane proteins probed by mass spectrometry J. Am. Chem. Soc., 137 (2015).

E.G. Bligh, W.J. Dyer A rapid method of total lipid extraction and purification Can. J. Biochem. Physiol., 37 (1959).

F. Dumas, M.C. Lebrun, J.F. TocanneIs the protein/lipid hydrophobic matching principle relevant to membrane organization and functions? FEBS Lett., 458 (1999).

T. Cajka, O. FiehnComprehensive analysis of lipids in biological systems by liquid chromatography-mass spectrometry TrAC Trends Anal. Chem., 61 (2014).

B. Fuchs, R. Süß, K. Teuber, M. Eibisch, J. SchillerLipid analysis by thin-layer chromatography-a review of the current state J. Chromatogr. A, 1218 (2011).

G. Guidotti Membrane proteins Annu. Rev. Biochem., 41 (1972).


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